Wang, Hongbo Wang, Xiaolong Li, Yan Yu, Hao Wang, Changliang Feng, Chunmei Xu, Guohui Chen, Jiajun You, Jiabin Wang, Pengfei Wu, Xu Zhao, Rui Zhang, Guohua

PMID:23977108Ĭhronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium. The present results provide a new insight into the potential mechanism of PCI-24781 in SK-N-DZ cell line. Selective knockdown of RuvBL2 rescued cells from PCI-24781-induced cell death, implying that RuvBL2 might play an important role in anti-tumor activity of PCI-24781 in SK-N-DZ cells. Western blotting confirmed the expression level of five candidate proteins including prohibitin, hHR23a, RuvBL2, TRAP1 and PDCD6IP. Totally 42 differentially expressed proteins were identified by MALDI-TOF MS system. Further proteomic analysis revealed differential protein expression profiles between non-treated and PCI-24781 treated SK-N-DZ cells. Treatment of SK-N-DZ with PCI-24781 also induced cell cycle arrest in G2/M phase and activated apoptosis signaling pathways via the up-regulation of DR4, p21, p53 and caspase 3. However, PCI-24781 caused the accumulation of acetylated histone H3 both in SK-N-DZ and HS-68 cell line. In the present study, we demonstrated that PCI-24781 treatment significantly inhibited tumor growth at very low doses in neuroblastoma cells SK-N-DZ, not in normal cell line HS-68. However, the underlying mechanisms of PCI-24781 are not clearly elucidated in neuroblastoma cells. PCI-24781 is a novel hydroxamic acid-based histone deacetylase (HDAC) inhibitor that has high efficacy and safety for cancer treatment. The survival rate among children with high-risk neuroblastoma is less than 40%, highlighting the urgent needs for new treatment strategies. Neuroblastoma is the second most common solid tumor diagnosed during infancy. Zhan, Qinglei Tsai, Sauna Lu, Yonghai Wang, Chunmei Kwan, Yiuwa Ngai, Saiming RuvBL2 Is Involved in Histone Deacetylase Inhibitor PCI-24781-Induced Cell Death in SK-N-DZ Neuroblastoma Cells

Our data suggest that SK-N-BE(2C cells have potential utility to serve as another model system to detect norepinephrine reuptake inhibition activity. The results obtained with the SK-N-BE(2C cells indicate that this model system can detect a range of ligand potencies, which compare well with other established transporter assays. Using this determined KM value, reuptake inhibition assays were then conducted with a variety of ligands including antidepressants, as well as piperazine and phenyltropane derivatives. Before conducting the assays, the SK-N-BE(2C cells were first evaluated for their ability to uptake norepinephrine and were shown to have a saturable uptake with a KM value of 416 nM. We propose that pomegranate is a potential nutritional strategy in slowing the progression of neurodegenerative disorders such as Alzheimer's disease.ĭevelopment of norepinephrine transporter reuptake inhibition assays using SK-N-BE(2C cellsĭirectory of Open Access Journals (Sweden)įull Text Available This report describes efforts to develop and validate novel norepinephrine transporter reuptake inhibition assays using human neuroblastoma SK-N-BE(2C cells in 24-well format. Phosphorylation of IÎ✫ and IKK was significantly (pÂ pomegranate inhibits inflammation, as well as amyloidogenesis in IL-1Î²-stimulated SK-N-SH cells. PWE (25-200Â Î¼g/ml) dose dependently reduced COX-2-dependent PGE2 production in SK-N-SH cells stimulated with IL-1Î². Levels of BACE-1 and AÎ² in SK-N-SH cells stimulated with IL-1Î² were measured with an in cell ELISA. Expression of COX-2, phospho-IÎ✫, and phospho-IKK proteins was evaluated, while NF-Î✫ reporter gene assay was carried out in TNFÎ±-stimulated HEK293 cells to determine the effect of PWE on NF-Î✫ transactivation. An enzyme immunoassay (EIA) was used to measure prostaglandin E2 (PGE2) production from supernatants of IL-1Î²-stimulated SK-N-SH cells. In this study, we aimed to assess the effects of freeze-dried pomegranate (PWE) on PGE2 production in IL-1Î²-stimulated SK-N-SH cells. (Punicaceae), and its constituents have been shown to inhibit inflammation. Velagapudi, Ravikanth Baco, Gina Khela, Sunjeet Okorji, Uchechukwu Olajide, Olumayokun Pomegranate inhibits neuroinflammation and amyloidogenesis in IL-1Î²-stimulated SK-N-SH cells.

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